Select from oYo-Link® Azide, Thiol, Tetrazine & DBCO
Perform click-reactions prior to antibody conjugation for optimal reaction conditions and a 10x increase in reaction efficiency.
oYo-Link® Click Chemistry Reagents enable the click reaction to take place prior to antibody labeling which results in highly efficient click reactions.
Simply mix oYo-Link with your desired antibody followed by photocrosslinking for conjugation in 30-sec hands on time, 2 hours total.
oYo-Link enables attachment of the labels to the heavy chains of the antibody and therefore ensures that the label does not interfere with antibody binding. Furthermore, site-specific labeling limits attachment to 1-2 labels per antibody, providing precise antibody-conjugate ratios.
Advantages of labeling with oYo-Link® Click Chemistry Reagents
- Perform click-reactions prior to antibody conjugation for optimal reaction conditions and a 10x increase in reaction efficiency.
- Site-specific labeling to the Fc-region every time guarantees no interference with antibody-binding
- Site-specific & covalent labeling results in uniform products and consistent, predictable results
- <30 seconds “hands-on” time, 2 hours total. Two simple steps, Mix and Illuminate
- Label as little as 1 µg of antibody per reaction in diluted antibody solutions, as low as 50 µg/mL
- Compatible with all common buffers, including BSA, Tris & Azide
oYo-Link® Click Chemistry & Chemical Conjugations
oYo-Link® Azide
Site-specifically label your antibody with oYo-Link® Azide for subsequent click chemistry reactions with DBCO-labeled molecules.
oYo-Link® DBCO
Site-specifically label your antibody with oYo-Link® DBCO for subsequent copper-free click chemistry reactions with Azide-labeled molecules.
oYo-Link® Tetrazine
Site-specifically label your antibody with oYo-Link® Tetrazine for subsequent click-chemistry with TCO-labeled molecules. Click below to learn more…
oYo-Link® Thiol
Site-specifically label your antibody with oYo-Link® Thiol for coupling reactions with Maleimide or reactions with other thiol-reactive agents. Click below to learn more…
Two steps, 30 seconds hands-on time, 100% sample recovery
Antibody labeling requires just two steps:
- Mix oYo-Link with your antibody
- Illuminate with non-damaging 365 nm UV light for <2 hours
The entire procedure requires just two steps and less than 30 seconds of hands-on time. Any unused oYo-Link reagent can be saved for later use.
How oYo-Link® Works
oYo-Link reagents consist of low molecular weight (~8 kDa), high-affinity antibody-binding domains that possess a photo-crosslinker within their Fc-binding site. Upon illumination with non-damaging Black-light, oYo-Link forms a covalent bond with the antibody. This procedure is referred to as Light-Activated Site-Specific Conjugation (LASIC). Any label that is attached to oYo-Link will be covalently attached to the desired antibody.
All that is needed to label your antibody is the oYo-Link reagent and our LED photo-crosslinking device to perform the conjugation, or a compatible device.
Watch our webinar to find out more about oYo-Link.
Looking to outsource your antibody conjugation?
AlphaThera also offers affordable, high quality antibody labeling performed by our expert team. Just $75 per conjugate plus the cost of reagents. Speak with our team about your project.
Talk to us about your project