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Read the LED PX Device user manual for instructions on how to use AlphaThera’s device to perform the photo-crosslinking for antibody conjugation.
Read our antibody conjugation usual manual for instructions on how to easily conjugate your antibody with oYo-Link products.
This protocol describes how to remove free oYo-Link from conjugated antibody-oYo-Link using oYo-Capture Magnetic beads.
Guides users on how to immobilize their antibodies in 96-well plates for subsequent antigen capture assays.
Guides users on how to perform Thiol-Maleimide coupling reactions with oYo-Link Thiol.
Guides users on how to perform click-chemistry coupling reactions with oYo-Link Azide.
Guides users on how to perform copper-free click-chemistry coupling reactions with oYo-Link DBCO.
Guides users on how to perform click-chemistry coupling reactions with oYo-Link Tetrazine.
Antibody-oligonucleotide conjugates are becoming increasingly popular to improve assay sensitivity and specificity, and for their potential in single-cell and subcellular analysis. However the conjugation process continues to be a barrier. In this eBook we look at the key applications, the challenges and limitations of oligo conjugation, and new technologies enabling easy labeling.
In this eBook we discuss how existing antibody labeling methods create a barrier for ADC development including limiting the high-throughput capability, and the uncertainties that arise from non-uniform antibody-drug conjugates. We also discuss the solution to overcome these barriers using site-specific conjugation that yield homogenous ADCs for rapid prototyping.
This video demonstrates how to install bulbs for the LEDPX2 crosslinking device. AlphaThera’s LED Photo-Crosslinking Device (LEDPX) uses non-damaging light (365nm) to enable the efficient and site-specific labeling of antibodies with all oYo-Link products.
Labeling your antibodies with Biotin, Enzymes, Oligos, Azide, and More just got easier. Try oYo-Link and ensure you attach cargo to the Fc-region of your antibody every time with our site-specific technology.