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Research Use Only.
oYo-Link® HRP enables the rapid production of highly uniform primary antibody-HRP conjugates in under 2 hours, with only 30 seconds hands-on time. Site-directed conjugation of 1-2 HRP labels to the heavy chain of compatible primary antibodies ensures consistent conjugates that do not require optimization. Simply mix the oYo-Link® HRP with your antibody and then illuminate using our LED Photocrosslinking device.
Requirements: Photo-Crosslinking Device
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oYo-Link® HRP allows rapid direct conjugation of compatible antibodies to HRP for a wide range of applications including ELISA, Western blotting and IHC.
Using directly labeled primary antibodies is a preferred detection method for these applications because of the many wash steps required in indirect detection, as well as the cross-species reactivity that can occur with the use of secondary antibodies. However labeling primary antibodies can be complex and time consuming.
oYo-Link® HRP allows researchers to directly conjugate their antibody to HRP, so there is no need for secondary antibodies. Furthermore, the labeling procedure is rapid and requires only 30 seconds hands-on time, with conjugates ready within 2 hours. The researcher simply has to mix their antibody with oYo-Link HRP and then illuminate for 2 hours using our LED Crosslinking device. oYo-Link is compatible with all common storage buffers so there is no need to purify or desalt your antibody prior to labeling. (see Buffer Compatibility Table)
oYo-Link® also enables site-specific labeling of the antibody heavy chain only. This ensures uniform labeling of 2 HRP labels per antibody, producing consistent antibody-HRP conjugates that require no further optimization steps, therefore saving time and cost.
Applications: Western Blotting, ELISA, IHC
Requirements: To label your compatible antibodies with oYo-Link®, you will need a light source emitting at 365nm (6-10W). Purchase AlphaThera’s LED Photo-Crosslinking Device here, or use a compatible device.
Placing an order: For credit card purchases, proceed with checkout on website. To request a quote click here.
Technical Support: For any technical questions please contact our technical support team here.
Read our antibody conjugation usual manual for instructions on how to easily conjugate your antibody with oYo-Link products.
Labeling your antibodies with Biotin, Enzymes, Oligos, Azide, and More just got easier. Try oYo-Link and ensure you attach cargo to the Fc-region of your antibody every time with our site-specific technology.
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|Catalog||Amount Labeled||Price(USD)||Spec Sheet|
|AT6002-100||Label 100 µg Antibody||$350||Download Here|
|AT6002-mIgG1-100||Label 100 µg mouse IgG1 Antibody||$350||Download Here|
|AT6002-500||Label 500 µg Antibody||$1500||Download Here|
|AT6002-mIgG1-500||Label 500 µg mouse IgG1 Antibody||$1500||Download Here|
|AT6002-1000||Label 1000 µg Antibody||$2500||Download Here|
|Product Name|| |
oYo-Link® Horseradish Peroxidase (HRP)
|Catalog #|| |
|Lot #|| |
See Product Spec Sheet shipped with product
|Antibody and Buffer Compatibility|| |
See oYo-Link Antibody & Buffer Compatibility Table here.
|Molecular Weight|| |
|Molar Concentration|| |
|Storage Buffer|| |
1 x PBS
|Shipping Condition|| |
This product is shipped as a dried clear or white pellet at ambient temperature.
Before opening, briefly centrifuge each tube to ensure that the entire pellet is at the bottom. For all product tubes: Add 100 µL sterile ddH2O and vortex tube briefly until fully dissolved.
|Storage and Stability|| |
The dried product can be stored at 4°C or -20°C upon receipt. Following reconstitution, the product can be stored at 4°C for short-term durations (< 1 week) and -20°C to -80°C for longer time-spans.
|Conjugation Instructions|| |
After reconstitution, each product tube is capable of labeling up to 100 µg of antibody. Please find and follow the Antibody Conjugation User Manual here.
|Other Notes|| |
Disposal of product should be performed in compliance with all applicable federal, state, and local regulations. This product does not meet hazard classification criteria based on evaluations made by our company under the OSHA Hazard Communication Standard, 29 CFR 1910.1200.
Please check the antibody compatibility table. Note: oYo-Link mIgG1 can be used to label mouse IgG1 antibody. Currently, oYo-Link mIgG1 is only available for certain products. Please check the product webpage. oYo-Link mIgG1 only labels mouse IgG1 and is not compatible with other antibodies.
Yes, since oYo-Link specifically bind to the heavy chain of IgG, it works in the presence of both albumin and Tris. oYo-Link is compatible with all common buffers. See the full buffer compatibility table here.
With the exception of oYo-Link MNase and oYo-Link DBCO, all oYo-Link products are shipped as white or clear pellets and are very stable. While we recommend cold storage, they will not exhibit any loss of activity if left at room temperature for a week. oYo-Link MNase and oYo-Link DBCO must be shipped and stored cold (-20°C) immediately upon arrival, otherwise there will be a loss in activity.
Yes. If you are interested in our conjugation service, please contact us at firstname.lastname@example.org. For custom conjugation services, you will need to send us your antibody. The conjugation and purification fee depends on the size and specifications of each order. Purification is not required for most applications.
For most subclasses and species of antibody, oYo-Link will result in the conjugation of 2 labels per antibody (maximum), one label per heavy chain. For example, ~95% of human IgG and Rabbit IgG will have two labels per antibody. However, there are a few antibodies such as mouse IgG2b and goat IgG, where the conjugation is slightly less efficient. In this case, 60 to 80% of the antibody will be labeled and have a mixture of 0, 1 and 2 labels per antibody. See the antibody conjugation efficiency here
Antibody conjugation can be checked on SDS-PAGE gel. An example is present in the supporting data on each product page.
The conjugated and unconjugated antibody binding affinities have been tested via both ELISA and cell binding assays and don’t show any difference.
For most immunoassay applications, purification is not necessary. In our standard recommended protocol, the molar ratio of oYo-Link-to-antibody is 5:1. These conditions ensure that the antibody conjugation efficiency reaches its maximum; however there will be a slight excess remaining in your antibody mixture. This is usually removed during immunoassay washing steps. However, if your application requires high antibody-conjugate purity, please contact us for more information on this process and for a detailed protocol.
When working with oYo-Link Azide (Catalog #: AT3002) and oYo-Link DBCO (Catalog #: AT3003), it is required to perform the click chemistry coupling reactions with the molecule of interest first, prior to photo-crosslinking to an antibody. This is to avoid UV illumination damage to the click moiety and to avoid diluting oYo-Link prior to the reaction to maximize the reaction efficiency. For oYo-Link Tetrazine (Catalog #: AT3004) and oYo-Link Thiol (Catalog #: AT3001); it is recommended to perform the coupling reaction before photo-crosslinking. This is to avoid diluting oYo-Link prior to the coupling reaction to maximize the reaction efficiency.
We recommend working with at least 1 µg of antibody; however, it is possible that even lower amounts of antibody can be labeled.
Yes, antibodies can be efficiently labeled even if diluted to concentrations as low as 50 µg/mL.
4 EPITOPE TAGS: Please select only 4 Tags Below:
Each tag will be able to label the amount of Ab you selected on the previous page.