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Two Steps, 30 Seconds Hands-on Time, 100% Sample Recovery
Antibody labeling requires just two steps:
1. Mix oYo-Link with your antibody
2. Illuminate with non-damaging 365 nm UV light for <2 hours
The entire procedure requires just two-steps and less than than 30 seconds of hands-on time. Any unused oYo-Link reagent can be saved for later use.
oYo-Link® vs Competitor Antibody Labeling Reagents & Kits
|Feature||oYo-Link®||Other Antibody Labeling Reagents|
|Antibody concentration requirement||>50ug/mL||1mg/mL|
|Buffer requirements||Almost no limit||No Tris, No BSA, No Glycerol etc|
|Stabality of regeants||Highly stable||Loss of activity after reconstitution|
|Single/multiple use||Multiple use||Single use|
|Stability of conjugated antibody||Highly Stable||May cause aggregation|
|Antibody binding affinity||Maintained||Lowered or lost|
|Verification of antibody conjugation||Run on SDS-PAGE gel||Hard to know|
|Removal of free reagents||No need in most applications||Removal required|
Wide Variety of Applications
The oYo-Link Antibody Labeling System can be used to site-specifically and covalently label the Fc region of almost any “off-the-shelf” antibody with biotin, drugs, click chemistry tags (e.g. azide, DBCO), epitope tags, enzymes (e.g. MNase), HyperBright™ fluorescent labels, oligonucleotides, and more.
Easy & rapid labeliong
Covalent, site-specific labeling to the Fc region
Label small amounts
Robust buffer compatibility
oYo-Link® Origins In Research
AlphaThera has a variety of publications linked to the origin of oYo-Link® technology.
LASIC Conjugation Technology
Protein Z & Site-Specific Conjugation
Site-specific labeling of Nanoparticles with IgG
1. Hui, J.Z., Tamsen, S., Song, Y., Tsourkas, A. (2015) LASIC: Light activated site-specific conjugation of native IgGs. Bioconjugate Chemistry, 26(8), 1456-1460. DOI: 10.1021/acs.bioconjchem.5b00275
2. Hui, J.Z., Tsourkas, A. (2014) Optimization of photo-active Protein Z for fast and efficient site-specific conjugation of native IgG. Bioconjugate Chemistry, 25(9), 1709-1719. DOI: 10.1021/bc500305v
3. Hui, J.Z., Al Zaki, A., Cheng, Z., McNitt, C.D., Popik, V., Zhang, H., Luning Prak, E.T., Tsourkas, A. (2014) Facile method for the site-specific, covalent attachment of full-length IgG onto nanoparticles. Small, 10(16), 3354-3363. DOI: 10.1002/smll.201303629