oYo-Link® SureLight® APC

Rapid, site-specific labeling of compatible antibodies with up to two APC labels. 30 seconds hands-on time, conjugates ready in just 2 hours.

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oYo-Link® SureLight® APC Antibody Labeling Reagents

For research use only.

oYo-Link® SureLight® APC enables site-specific labeling of compatible antibodies with up to two labels of APC (allophycocyanin) for flow cytometry/FACS and T-cell labeling.

Simply mix the oYo-Link SureLight APC with the desired antibody followed by photocrosslinking for conjugation in 30-sec hands on time, 2 hours total. 

Requirements: Photo-Crosslinking Device

Quote or Bulk Orders? Click here.

This product is manufactured using SureLight® allophycocyanin which is a product of Columbia Biosciences.

APC is an ultra-sensitive phycobiliprotein that exhibits far-red fluorescence (absorbs at 653nm and emits at 660nm). The intense brightness of APC means it is popular for flow cytometry/FACS and T-cell labeling.

This product is manufactured using SureLight® Allophycocyanin which is a product of Columbia Biosciences, and uses a proprietary non-destructive cross-linking process that increases the thermal stability while maintaining fluorescent and spectral integrity of the native dye.

Generate antibody-APC conjugates in only 30-seconds hands on time, 2 hours total.

The antibody labeling procedure simply requires mixing oYo-Link® SureLight® APC with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm) for 2 hours. 

Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table).

Excitation/Emission: 653nm/660nm

Applications: Flow Cytometry/FACS, T-cell labeling.

Requirements: To label your compatible antibodies with oYo-Link®, you will need a light source emitting at 365nm (6-10W). Purchase AlphaThera’s LED Photo-Crosslinking Device here, or use a compatible device.

Placing an order: For credit card purchases, proceed with checkout on website. To request a quote click here

Technical Support: For any technical questions please contact our technical support team here

Webinar: Simple, fast, site-specific antibody labeling with oYo Link®

Watch our short introduction to AlphaThera’s oYo-Link® site-specific antibody conjugation technology and learn how to streamline your assays with these superior labeling products.

Antibody Conjugation User Manual

Read our antibody conjugation usual manual for instructions on how to easily conjugate your antibody with oYo-Link products.

Demo: oYo-Link® Antibody Conjugation

Find out how to site-specifically label your antibody with just two steps: mix and illuminate. 30 seconds hands-on time, 2 hours total.

oYo-Link® Product Brochure

Download our product brochure

oYo-Link® SureLight® APC Flow Cytometry Data

SPR and BD FACS Canto II, A431 Cells, conjugates tested at 100 ng/test

 

Catalog Amount Labeled Price(USD) Spec Sheet
ATSL002-100 Labels 100 µg Antibody $350 Download Here
ATSL002-mIgG1-100 Label 100 µg mouse IgG1 Antibody $350 Download Here
ATSL002-500 Label 500 µg Antibody $1250 Download Here
ATSL002-mIgG1-500 Label 500 µg mouse IgG1 Antibody $1250 Download Here
ATSL002-1000 Label 1000 µg Antibody $1875 Download Here
ATSL002-mIgG1-1000 Label 1000 µg mouse IgG1 Antibody $1875 Download Here

Specifications:

Product Name

oYo-Link® APC

Catalog #

ATSL002

Lot #

See Product Spec Sheet shipped with product.

Antibody and Buffer Compatibility

See oYo-Link Antibody & Buffer Compatibility Table here.

Molecular Weight

54k/da

Concentration

1.8mg/mL

Molar Concentration

33 µM

Storage Buffer

1 x PBS

Shipping Condition

This product is shipped as blue liquid with cold gel packs.

Reconstitution

No reconstitution required. Before opening, briefly centrifuge each tube to

ensure that the entire solution is at the bottom.

Storage and Stability

The product should be stored at 2-8°C in the dark. Do not freeze.

Conjugation Instructions

After reconstitution, each product tube is capable of labeling up to 100 µg of antibody. Please find and follow the Antibody Conjugation User Manual here.

Dye Lot #

APC000-24-125

(from Columbia Biosciences)

Fluorescence

Peak excitation (approximate): 653 nm

Peak emission (approximate): 660 nm

Other Notes

Disposal of product should be performed in compliance with all applicable federal, state, and local regulations. This product does not meet hazard classification criteria based on evaluations made by our company under the OSHA Hazard Communication Standard, 29 CFR 1910.1200.

Will oYo-Link work with my antibody?

Please check the antibody compatibility table. Note: oYo-Link mIgG1 can be used to label mouse IgG1 antibody. Currently, oYo-Link mIgG1 is only available for certain products. Please check the product webpage. oYo-Link mIgG1 only labels mouse IgG1 and is not compatible with other antibodies.

Can oYo-Link be used to label antibodies in the presence of albumin and/or Tris?

Yes, since oYo-Link specifically bind to the heavy chain of IgG, it works in the presence of both albumin and Tris. oYo-Link is compatible with all common buffers. See the full buffer compatibility table here.

If an oYo-Link product has accidentally been left at room temperature for a week, will it still work?

With the exception of oYo-Link DBCO, all oYo-Link products are shipped as white or clear pellets and are very stable. While we recommend cold storage, they will not exhibit any loss of activity if left at room temperature for a week. oYo-Link DBCO must be shipped and stored cold (-20°C) immediately upon arrival, otherwise there will be a loss in activity.

Does AlphaThera offer conjugation service?

Yes. You can find more information about our conjugation services here.

How many oYo-Link labels will be conjugated to each antibody?

For most subclasses and species of antibody, oYo-Link will result in the conjugation of 2 labels per antibody (maximum), one label per heavy chain. For example, ~95% of human IgG and Rabbit IgG will have two labels per antibody. However, there are a few antibodies such as mouse IgG2b and goat IgG, where the conjugation is slightly less efficient. In this case, 60 to 80% of the antibody will be labeled and have a mixture of 0, 1 and 2 labels per antibody. See the antibody conjugation efficiency here

How do I know if oYo-Link was successfully conjugated to my antibody?

Antibody conjugation can be checked on SDS-PAGE gel. An example is present in the supporting data on each product page.

Does 365nm UV illumination damage the antibody and its binding affinity?

The conjugated and unconjugated antibody binding affinities have been tested via both ELISA and cell binding assays and don’t show any difference.

Do I need to remove free oYo-Link after conjugating to antibody?

For most immunoassay applications, purification is not necessary. In our standard recommended protocol, the molar ratio of oYo-Link-to-antibody is 5:1. These conditions ensure that the antibody conjugation efficiency reaches its maximum; however there will be a slight excess remaining in your antibody mixture. This is usually removed during immunoassay washing steps. However, if your application requires high antibody-conjugate purity, please contact us for more information on this process and for a detailed protocol.

What is the lowest amount of antibody that can be labeled by oYo-Link reagents?

We recommend working with at least 1 µg of antibody; however, it is possible that even lower amounts of antibody can be labeled.

Can antibodies that are highly dilute be labeled by oYo-Link reagents?

Yes, antibodies can be efficiently labeled even if diluted to concentrations as low as 50 µg/mL.

oYo-Link® SureLight® APC Benefits

  • Rapid & easy labeling, only 30 seconds of hands-on time, 2 hours total conjugation.
  • Label primary antibodies for direct detection, avoid laborious wash steps and cross-species reactivity.
  • Site-specific labeling at the antibody Fc region guarantees no interference with the antigen binding site.
  • Site-specific labeling ensures the production of highly consistent conjugates with no requirement for further optimization.
  • Label from as little as 1 µg and work with antibody concentrations as low as 50 µg/mL. No need to concentrate or centrifuge your sample prior to labeling.
  • Compatible with all common buffers – no need for antibody purification.