Antibody Conjugation Service
We provide site-specific antibody conjugation services including oligonucleotide, fluorescent, enzyme, and site-specific labeling using our proprietary oYo-Link technology.
Choose from our oYo-Link® range of labels and our expert conjugation scientists will covalently and site-specifically conjugate your antibody, and purify conjugates post-labeling if required, ensuring you receive the highest quality conjugates that meet your specific experimental requirements. View our FAQs here for more information.
Pricing:
-
Only $75 per conjugate + the cost of reagents.
-
Purification (FPLC): $250 per conjugate (bulk orders may incur an additional charge)
Our Antibody Conjugation Service
-
Only $75 per conjugate plus the cost of reagents
-
All conjugates validated by our expert team
-
Optional additional purification
-
Flexible to accommodate a wide range of scale requirements
-
We accept antibody samples as dilute as 50 ug/mL
-
Highest quality, site-specific conjugates
Types of Conjugation Available
We offer a range of antibody labeling services including antibody oligonucleotide conjugation, antibody-drug conjugation, fluorescent labeling, enzyme conjugation, biotin labeling and more. Contact us to tell us more about your project requirements.
Speak to our team to discuss your project
Our team will be in touch within 48 hours
Frequently Asked Questions
A: Yes, our technical support team is available to discuss your project with you. Please contact us here.
A: Antibodies can be shipped according to the vendor’s instruction to:
AlphaThera Inc.
3675 Market St
Suite 200
Philadelphia, PA 19104
Please note that we will require an additional 10 µg of antibody for QC purposes.
AlphaThera Inc.
3675 Market St
Suite 200
Philadelphia, PA 19104
Please note that we will require an additional 10 µg of antibody for QC purposes.
A: For most subclasses and species of antibody, oYo-Link will result in the conjugation of ~2 labels per antibody; one per heavy chain. For example, ~95% of human IgG and Rabbit IgG will have two labels per antibody. However, there are a few antibodies, such as mouse IgG2b and goat IgG, which may result in slightly less efficient conjugation. In this case, 60 to 80% of the antibody will be labeled with 1 or 2 labels per antibody. View our antibody conjugation efficiency table herefor more information.
A: Our conjugation service is $75 per antibody plus the cost of reagents. Purification (FPLC) is available for $250 per conjugate. Please note that bulk orders may incur an additional charge.
A: There are no predefined upper or lower quantity limits for conjugation service, providing flexibility to accommodate a wide range of scale requirements. However, for purification services, the minimum input amount is 25 µg when using oYo-Capture Magnetic Beads and 100 µg when using FPLC purification.
A: Antibody samples as dilute as 50 µg/mL are accepted.
A: Purification of conjugates is available for an additional fee, which will be provided upon request.
Two types of purification are available:
1. Purification using oYo-Capture Magnetic Beads for the removal of free oYo-Link, which can help reduce assay background; this requires a minimum of 25 µg of antibody.
2. Size exclusion chromatography; this requires a minimum of 100 ug of antibody.
For extremely sensitive assays requiring the highest purity of conjugated antibodies, FPLC purification is recommended, and if applied, the chromatography data will be included. FPLC purification is available for $250 per conjugate.
Two types of purification are available:
1. Purification using oYo-Capture Magnetic Beads for the removal of free oYo-Link, which can help reduce assay background; this requires a minimum of 25 µg of antibody.
2. Size exclusion chromatography; this requires a minimum of 100 ug of antibody.
For extremely sensitive assays requiring the highest purity of conjugated antibodies, FPLC purification is recommended, and if applied, the chromatography data will be included. FPLC purification is available for $250 per conjugate.
A: The estimated lead time is 2–3 weeks after receipt of all required samples, including your antibodies and, in the case of custom oYo-oligo products, the oligo(s) supplied by IDT.
A: The QC data for antibody conjugation will be provided as an SDS-PAGE Gel of unconjugated and conjugated antibody. Additional QC data can be provided upon request.