Select from oYo-Link® SureLight® APC, Far Red APC, PE, PE-CY7
oYo-Link® SureLight® fluorescent antibody labeling reagents enable the covalent, site-specific labeling of primary antibodies to fluorescent proteins. Site-specific labeling at the antibody heavy chain ensures no interference with antibody binding, producing highly homogenous conjugates with no need to optimize labeling conditions. Select from APC, Far Red APC, PE and PE-CY7.
oYo-Link® SureLight® is manufactured using high quality PE & APC proteins manufactured by Columbia Biosciences. They use a proprietary non-destructive cross-linking process that increases thermal stability while maintaining fluorescent and spectral integrity of the native protein.
Advantages of labeling with oYo-Link® SureLight®
- Directly label primary antibodies with only 30s hands-on time, 2 hours total
- Label primary antibodies, avoid laborious wash steps and cross-species reactivity
- Site-specific, covalent labeling guarantees no interference with antibody performance
- Compatible with all common buffers, even those containing Tris, azide & BSA
- Highly homogeneous – no need to optimize conditions for each new antibody
- Label from as little as 1 µg and work with antibody concentrations as low as 50 µg/mL
- Store any unused reagents for later
oYo-Link® SureLight® Fluorescent Proteins
oYo-Link® SureLight® PE
Exc/Emm: 565nm/574nm
Rapid, site-specific labeling of compatible antibodies with up to two PE labels. 30 seconds hands-on time, conjugates ready in just 2 hours.
oYo-Link® SureLight® APC
Exc/Emm: 653nm/660nm
Rapid, site-specific labeling of compatible antibodies with up to two APC labels. 30 seconds hands-on time, conjugates ready in just 2 hours.
oYo-Link® SureLight® Far Red APC
Exc/Emm: 713nm/718nm
Rapid, site-specific labeling of compatible antibodies with up to two Far Red APC labels. 30 seconds hands-on time, conjugates ready in just 2 hours.
oYo-Link® SureLight® PE-Cy7
Exc/Emm: 488 nm / 572 nm, 776 nm
Rapid, site-specific labeling of compatible antibodies with up to two PE-Cy7 labels. 30 seconds hands-on time, conjugates ready in just 2 hours.
Two steps, 30 seconds hands-on time, 100% sample recovery
Antibody labeling requires just two steps:
- Mix oYo-Link with your antibody
- Illuminate with non-damaging 365 nm UV light for <2 hours
The entire procedure requires just two steps and less than 30 seconds of hands-on time. Any unused oYo-Link reagent can be saved for later use.
How oYo-Link® Works
oYo-Link reagents consist of low molecular weight (~8 kDa), high-affinity antibody-binding domains that possess a photo-crosslinker within their Fc-binding site. Upon illumination with non-damaging Black-light, oYo-Link forms a covalent bond with the antibody. This procedure is referred to as Light-Activated Site-Specific Conjugation (LASIC). Any label that is attached to oYo-Link will be covalently attached to the desired antibody.
All that is needed to label your antibody is the oYo-Link reagent and our LED photo-crosslinking device to perform the conjugation, or a compatible device.
Watch our webinar to find out more about oYo-Link.
Looking to outsource your antibody conjugation?
AlphaThera also offers affordable, high quality antibody labeling performed by our expert team. Just $75 per conjugate plus the cost of reagents. Speak with our team about your project.
Talk to us about your project