oYo-Link® SureLight® PE-Cy7

Rapid, site-specific labeling of compatible antibodies with up to two PE-Cy7 labels. 30 seconds hands-on time, conjugates ready in just 2 hours.

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oYo-Link® SureLight® PE-Cy7 Antibody Labeling Reagents

For research use only.

Streamline your antibody labeling with oYo-Link® SureLight® PE-Cy7 antibody labeling reagents. oYo-Link® SureLight® PE-Cy7 enables rapid, site-specific labeling of compatible antibodies with up to two labels of PE-Cy7. Labeled antibodies can be used in applications such as flow cytometry, western blot, ELISA, and IHC.

With just 2 easy steps and 30 seconds hands-on time, you can achieve high-quality conjugates. Simply mix the oYo-Link® SureLight® PE-Cy7 with the desired antibody followed by photocrosslinking for 2 hours. oYo-Link® SureLight® PE-Cy7 is compatible with all common buffers so there is no requirement for antibody purification prior to conjugation.

Requirements: Photo-Crosslinking Device

Quote or Bulk Orders? Click here.

SureLight® PE-Cy7 is supplied by Columbia Biosciences.

oYo-Link SureLight fluorescent protein labeling reagents absorption emissions spectra
oYo-Link® SureLight® PE-Cy7
oYo-Link® SureLight® PE-Cy7

PE-Cy7 is a far red-emitting tandem fluorophore composed of R-phycoerythrin (PE) and cyanine dye Cy7, allowing for detection of fluorescence at longer wavelengths (around 780nm)

This product is manufactured using SureLight® Pe-Cy7, a product of Columbia Biosciences.

Generate antibody-PE-Cy7 conjugates in only 30-seconds hands on time, 2 hours total.

The antibody labeling procedure simply requires mixing oYo-Link® SureLight® PE-Cy7 with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). 

Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table).

Applications: For fluorescence-based detection. Applications that do not require photostability.

Excitation/Emission (approximate): 488 nm / 572 nm, 776 nm

Requirements: To label your compatible antibodies with oYo-Link®, you will need a light source emitting at 365nm (6-10W). Purchase AlphaThera’s LED Photo-Crosslinking Device here, or use a compatible device.

Placing an order: For credit card purchases, proceed with checkout on website. To request a quote click here. 

Technical Support: For any technical questions please contact our technical support team here. 

oYo-Link® SureLight® PE-Cy7 Cetuximab Conjugates

Flow Cytometry analysis of EGFR expression on human A431 cell line.

A431 human epidermoid carcinoma cells were either:

  1. Stained with Rituximab conjugated to the indicated fluorophore (open histogram)
  2. Stained with Cetuximab conjugated (shaded histogram)  

Median Fluorescent Intensity (MFI)

Rituximab: 36.16

Cetuximab: 48,962.90

Specifications:

Product Name

oYo-Link® SureLight® PE-Cy7
Catalog #

ATSL003
Lot #

See the product spec sheet shipped with the product.
Antibody and buffer compatibility

View the oYo-Link Antibody & Buffer Compatibility Table here.

Note: To label mouse IgG1 antibody, you must order the oYo-Link mIgG1 products, which will only label mIgG1 antibody.
Molar Concentration

33 µM
Storage Buffer

1 x PBS
Shipping Condition

This product is shipped as red liquid with cold gel packs.
Reconstitution

No reconstitution required. Before opening, briefly centrifuge each tube to ensure that the entire solution is at the bottom.
Storage and Stability

The product should be stored at 2-8°C in the dark. Do not freeze.
Fluorescence

Peak excitation (approximate): 488 nm

Peak emission (approximate): 572 nm, 776 nm
Other Notes

Disposal of product should be performed in compliance with all applicable federal, state, and local regulations. This product does not meet hazard classification criteria based on evaluations made by our company under the OSHA Hazard Communication Standard, 29 CFR 1910.1200.

Will oYo-Link work with my antibody?

Please check the antibody compatibility table. Note: oYo-Link mIgG1 can be used to label mouse IgG1 antibody. Currently, oYo-Link mIgG1 is only available for certain products. Please check the product webpage. oYo-Link mIgG1 only labels mouse IgG1 and is not compatible with other antibodies.

Can oYo-Link be used to label antibodies in the presence of albumin and/or Tris?

Yes, since oYo-Link specifically bind to the heavy chain of IgG, it works in the presence of both albumin and Tris. oYo-Link is compatible with all common buffers. See the full buffer compatibility table here.

If an oYo-Link product has accidentally been left at room temperature for a week, will it still work?

With the exception of oYo-Link DBCO, all oYo-Link products are shipped as white or clear pellets and are very stable. While we recommend cold storage, they will not exhibit any loss of activity if left at room temperature for a week. oYo-Link DBCO must be shipped and stored cold (-20°C) immediately upon arrival, otherwise there will be a loss in activity.

Does AlphaThera offer conjugation services?

Yes. You can find more information about our conjugation services here.

How many oYo-Link labels will be conjugated to each antibody?

For most subclasses and species of antibody, oYo-Link will result in the conjugation of 2 labels per antibody (maximum), one label per heavy chain. For example, ~95% of human IgG and Rabbit IgG will have two labels per antibody. However, there are a few antibodies such as mouse IgG2b and goat IgG, where the conjugation is slightly less efficient. In this case, 60 to 80% of the antibody will be labeled and have a mixture of 0, 1 and 2 labels per antibody. See the antibody conjugation efficiency here

How do I know if oYo-Link was successfully conjugated to my antibody?

Antibody conjugation can be checked on SDS-PAGE gel. An example is present in the supporting data on each product page.

Does 365nm UV illumination damage the antibody and its binding affinity?

The conjugated and unconjugated antibody binding affinities have been tested via both ELISA and cell binding assays and don’t show any difference.

Do I need to remove free oYo-Link after conjugating to antibody?

For most immunoassay applications, purification is not necessary. In our standard recommended protocol, the molar ratio of oYo-Link-to-antibody is 5:1. These conditions ensure that the antibody conjugation efficiency reaches its maximum; however there will be a slight excess remaining in your antibody mixture. This is usually removed during immunoassay washing steps. However, if your application requires high antibody-conjugate purity, please contact us for more information on this process and for a detailed protocol.

What is the lowest amount of antibody that can be labeled by oYo-Link reagents?

We recommend working with at least 1 µg of antibody; however, it is possible that even lower amounts of antibody can be labeled.

Can antibodies that are highly dilute be labeled by oYo-Link reagents?

Yes, antibodies can be efficiently labeled even if diluted to concentrations as low as 50 µg/mL.

oYo-Link® SureLight® PE-Cy7

  • Rapid & easy labeling, only 30 seconds of hands-on time, 2 hours total conjugation.
  • Label primary antibodies for direct detection, avoid laborious wash steps and cross-species reactivity.
  • Site-specific labeling at the antibody Fc region guarantees no interference with the antigen binding site.
  • Site-specific labeling ensures the production of highly consistent conjugates with no requirement for further optimization.
  • Label from as little as 1 µg and work with antibody concentrations as low as 50 µg/mL. No need to concentrate or centrifuge your sample prior to labeling.
  • Compatible with all common buffers – no need for antibody purification.

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