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Affordable, high quality conjugation to HRP, performed by our expert team
Don’t waste valuable time on complex antibody conjugations. Our expert team of conjugation scientists will harness the power of AlphaThera’s oYo-Link Site-Specific labeling technology to provide you with the highest quality antibody conjugates that meet your specific experimental requirements.
Our unique technology combined with our rigorous processes enables maximum conjugation efficiency, limiting excess unconjugated label and antibody, and ensuring reliability and accuracy. For researchers carrying out highly sensitive assays, we offer an additional purification services.
What to Expect
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Only $75 per conjugate plus the cost of reagents
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All conjugates validated by our expert team
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Optional additional purification
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Label as little as 100 ug of antibody
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We accept antibody samples as dilute as 50 ug/mL
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Highest quality, site-specific antibody conjugates
Our Process
Receive the highest quality conjugates
AlphaThera’s unique site-specific and covalent labeling approach ensures high quality conjugates with over 90% conjugation efficiency as well as consistency with the number of reporter molecules attached for controlled reactions.
We also offer an additional purification service for the removal of any unconjugated antibody or free label to guarantee reliable results in your assay.
Get Started
Tell us about your project requirements. One of our expert conjugation scientists will be in touch within 48 hours.
Frequently Asked Questions
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Yes, our technical support team is available to discuss your project with you. Please contact us here.
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Antibodies can be shipped according to the vendor’s instructions to:
AlphaThera Inc.
3401 Grays Ferry Ave
Pennovation Lab Building 250
Philadelphia, PA 19146
Please note that 3-5 ug will be used for QC.
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For most subclasses and species of antibody, oYo-Link will result in the conjugation of ~2 labels per antibody; one per heavy chain. For example, ~95% of human IgG and Rabbit IgG will have two labels per antibody. However, there are a few antibodies, such as mouse IgG2b and goat IgG, which may result in slightly less efficient conjugation. In this case, 60 to 80% of the antibody will be labeled with 1 or 2 labels per antibody. View our antibody conjugation efficiency table here for more information.
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For the 1st antibody, the fee is $75 plus the cost of reagents. FPLC purification is available for $250 per conjugate.
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We can offer labeling between 100ug to 3mg of antibody. If you require labeling of cargos (with the exception of oligo) to antibody quantities below 100ug, this will be charged at the rate of labeling 100ug.
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Purification of conjugates is available for an additional fee, which will be provided upon request. Two types of purification are available: 1. Purification using oYo-Capture Magnetic Beads 2. Size exclusion chromatography; this requires a minimum of 100 ug of antibody.
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Upon receipt of your antibody, conjugation and shipping of your conjugates will take 2-3 days.
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The QC data for antibody conjugation will be provided as an SDS-PAGE Gel of unconjugated and conjugated antibody. Additional QC data can be provided upon request.