oYo-Link® SureLight® PE

FOR RESEARCH USE ONLY: Rapid, site-specific labeling of compatible antibodies with up to two PE labels. 30 seconds hands-on time, conjugates ready in just 2 hours.

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oYo-Link® SureLight® PE Antibody Labeling Reagents

For research use only.

oYo-Link® SureLight® PE antibody labeling reagents enable site-specific conjugation of compatible antibodies with up to two labels of PE for FACS, flow cytometry, and multimer/tetramer applications.

Simply mix the oYo-Link® SureLight® PE with the desired antibody followed by photocrosslinking for conjugation in 30-sec hands on time, 2 hours total. Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see our Buffer Compatibility Table).

Additional Information

PE is an ultra-bright fluorophore isolated from a group of red photosynthetic pigments found primarily in red algae that emits an intensely bright red-orange fluorescence. PE conjugates are most commonly used for applications requiring high sensitivity such as flow cytometry, ELISAs and microarray assays. 

This product is manufactured using SureLight® RPE which is a product of Columbia Biosciences, and uses a proprietary non-destructive cross-linking process that increases the thermal stability while maintaining fluorescent and spectral integrity of the native dye.

Applications: For fluorescence-based detection. Applications that do not require photostability.

Excitation/Emission: 565nm/574nm

Requirements: To label your compatible antibodies with oYo-Link®, you will need a light source emitting at 365nm (6-10W). Purchase AlphaThera’s LED Photo-Crosslinking Device here, or use a compatible device.

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oYo-Link® SureLight® PE

oYo-Link® Protocol - Quick Reference Guide

Download this oYo-Link® Protocol/Quick Reference Guide for instructions on how to site-specifically label your antibody with oYo-Link®.

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Video: FAQ - What is the labeling efficiency of oYo-Link®?

FAQ: What is the conjugation efficiency of oYo-Link® Site Specific Antibody Labeling Reagents? For most subclasses and species of antibody, oYo-Link® will result in conjugation of 2 labels per antibody, therefore achieving approximately 100% labeled IgG. However, there are a few antibodies where the conjugation is slightly less efficient.

 

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Webinar: Simple, fast, site-specific antibody labeling with oYo Link®

Watch our short introduction to AlphaThera’s oYo-Link® site-specific antibody conjugation technology and learn how to streamline your assays with these superior labeling products.

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Antibody Conjugation User Manual

Read our antibody conjugation usual manual for instructions on how to easily conjugate your antibody with oYo-Link products.

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Demo: oYo-Link® Antibody Conjugation

Find out how to site-specifically label your antibody with just two steps: mix and illuminate. 30 seconds hands-on time, 2 hours total.

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oYo-Link® Product Brochure

Download our brochure for more information and to access 20% off your first order of oYo-Link® Antibody Labeling products.

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oYo-Link® SureLight® PE Flow Cytometry Data

SPR and BD FACS Canto II, A431 Cells, conjugates tested at 100 ng/test

Specifications:

Product Name

oYo-Link® SureLight® PE
Catalog #

ATSL001
Lot #

See the product spec sheet shipped with the product.
Antibody and Buffer Compatibility

View the oYo-Link Antibody & Buffer Compatibility Table here.

Note: To label mouse IgG1 antibody, you must order the oYo-Link mIgG1 products, which will only label mIgG1 antibody.
Molar Concentration

33 µM
Storage Buffer

1 x PBS
Shipping Condition

This product is shipped as red liquid with cold gel packs.
Reconstitution

No reconstitution required. Before opening, briefly centrifuge each tube to ensure that the entire solution is at the bottom.
Storage and Stability

The product should be stored at 2-8°C in the dark. Do not freeze.
Dye Lot #

RPE3000-24-039

(from Columbia Biosciences)
Fluorescence

Peak excitation (approximate): 565 nm

Peak emission (approximate): 574 nm
Other Notes

Disposal of product should be performed in compliance with all applicable federal, state, and local regulations. This product does not meet hazard classification criteria based on evaluations made by our company under the OSHA Hazard Communication Standard, 29 CFR 1910.1200.

Will oYo-Link work with my antibody?

Please check the antibody compatibility table. Note: oYo-Link mIgG1 can be used to label mouse IgG1 antibody. Currently, oYo-Link mIgG1 is only available for certain products. Please check the product webpage. oYo-Link mIgG1 only labels mouse IgG1 and is not compatible with other antibodies.

Can oYo-Link be used to label antibodies in the presence of albumin and/or Tris?

Yes, since oYo-Link specifically bind to the heavy chain of IgG, it works in the presence of both albumin and Tris. oYo-Link is compatible with all common buffers. See the full buffer compatibility table here.

If an oYo-Link product has accidentally been left at room temperature for a week, will it still work?

With the exception of oYo-Link® DBCO, oYo-Link® Thiol, oYo-Link® SureLight® APC, Far-Red APC, PE, and PE-Cy7, all oYo-Link products are shipped as white or clear pellets and are very stable. While we recommend cold storage, they will not exhibit any loss of activity if left at room temperature for a week.

oYo-Link DBCO must be shipped and stored cold (-20°C) immediately upon arrival, otherwise there will be a loss in activity.

oYo-Link® SureLight® APC, Far-Red APC, PE, and PE-Cy7 are shipped as liquid with cold gel packs and should be stored at 2-8°C in the dark. Do not freeze.

Does AlphaThera offer conjugation service?

Yes. We offer a conjugation service for $75 per antibody plus the cost of reagents. Purification (FPLC) is available for $250 per conjugate (please note that bulk orders may incur an additional charge).
You can find out more about our conjugation service here. For conjugation services, you will need to send us your antibody.

How many oYo-Link labels will be conjugated to each antibody?

For most subclasses and species of antibody, oYo-Link will result in the conjugation of 2 labels per antibody (maximum), one label per heavy chain. For example, ~95% of human IgG and Rabbit IgG will have two labels per antibody. However, there are a few antibodies such as mouse IgG2b and goat IgG, where the conjugation is slightly less efficient. In this case, 60 to 80% of the antibody will be labeled and have a mixture of 0, 1 and 2 labels per antibody. See the antibody conjugation efficiency here

How do I know if oYo-Link was successfully conjugated to my antibody?

Antibody conjugation can be checked on SDS-PAGE gel. An example is present in the supporting data on each product page.

Does 365nm UV illumination damage the antibody and its binding affinity?

The conjugated and unconjugated antibody binding affinities have been tested via both ELISA and cell binding assays and don’t show any difference.

Do I need to remove free oYo-Link after conjugating to antibody?

For most immunoassay applications, purification is not necessary. In our standard recommended protocol, the molar ratio of oYo-Link-to-antibody is 5:1. These conditions ensure that the antibody conjugation efficiency reaches its maximum; however there will be a slight excess remaining in your antibody mixture. This is usually removed during immunoassay washing steps. However, if your application requires high antibody-conjugate purity, please contact us for more information on this process and for a detailed protocol.

What is the lowest amount of antibody that can be labeled by oYo-Link reagents?

We recommend working with at least 1 µg of antibody; however, it is possible that even lower amounts of antibody can be labeled.

Can antibodies that are highly dilute be labeled by oYo-Link reagents?

Yes, antibodies can be efficiently labeled even if diluted to concentrations as low as 50 µg/mL.

oYo-Link® SureLight® PE

  • Rapid & easy labeling, only 30 seconds of hands-on time, 2 hours total conjugation.
  • Label primary antibodies for direct detection, avoid laborious wash steps and cross-species reactivity.
  • Site-specific labeling at the antibody Fc region guarantees no interference with the antigen binding site.
  • Site-specific labeling ensures the production of highly consistent conjugates with no requirement for further optimization.
  • Label from as little as 1 µg and work with antibody concentrations as low as 50 µg/mL. No need to concentrate or centrifuge your sample prior to labeling.
  • Compatible with all common buffers – no need for antibody purification.

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