oYo-Capture™ Magnetic Beads

For the rapid removal of free oYo-Link after antibody conjugation to ensure low background in high sensitivity assays.

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oYo-CaptureTM Magnetic Beads enable the efficient removal of free oYo-Link after antibody conjugation to ensure low background in high sensitivity assays. oYo-Capture enables the purification of up to 1mg of conjugated antibody.

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For Research Use Only.

oYo-Capture™ Magnetic beads provide a an efficient method of removing free oYo-Link® reagents post antibody conjugation to ensure low background in high sensitivity assays.

oYo-Link technology enables highly efficient labeling reactions, resulting in only a small excess of free oYo-Link post conjugation. However, for researchers carrying out highly sensitive assays, oYo-Capture enables the quick and easy removal of unconjugated oYo-Link in only 5 minutes, providing reassurance that there will be low assay background. Furthermore, oYo-Capture ensures excellent recovery of materials, so there is no risk of wasting precious antibody conjugate in the purification process.

Requirements: To use oYo-Capture Magnetic Beads you will need a magnetic stand.

Purchasing Information: For credit card purchases, proceed with checkout on website. Or request a quote here.


Technical Support: For technical questions contact us here.

Antibody-oYo-Link® Conjugate Purification Protocol

This protocol describes how to remove free oYo-Link from conjugated antibody-oYo-Link using oYo-Capture Magnetic beads.

oYo-Link® Product Brochure

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Free oYo-Link removal with oYo-Capture

Figure 1. Shows the absence of oYo-Link Azide-TAMRA after removal with oYo-Capture for only 5 minutes.

oYo-Capture Magnetic Beads Antibody Recovery Data.

Figure 2. oYo-Capture Magnetic Beads Antibody Recovery Data. 25 ug human IgG1-oYo-Link was mixed and captured by 100 uL of oYo-Capture beads (10 mg/mL concentration). As shown on the gel, the antibody heavy chain remains largely unchanged after purification (Lane 4 to Lane 5), indicating excellent recovery of materials.

Catalog Amount Labeled Price(USD) Spec Sheet
AT8101-10 Purifies up to 1mg of conjugated antibody $350 Download Here

Specifications:

  • Ensure low background in high sensitivity assays
  • Rapid removal free oYo-Link in only 5 minutes
  • Ensures excellent recovery of antibody conjugates