Fluorescent Protein Labeling
oYo-Link® SureLight®

Rapid labeling of antibodies to high quality fluorescent proteins

Select from oYo-Link® SureLight® APC, Far Red APC, PE, PE-CY7

oYo-Link® SureLight® reagents enable the covalent, site-specific labeling of primary antibodies to fluorescent proteins. Site-specific labeling at the antibody heavy chain ensures no interference with antibody binding, producing highly homogenous conjugates with no need to optimize labeling conditions.

oYo-Link® SureLight® is manufactured using high quality PE & APC proteins manufactured by Columbia Biosciences. They use a proprietary non-destructive cross-linking process that increases thermal stability while maintaining fluorescent and spectral integrity of the native protein.

Advantages of labeling with oYo-Link® SureLight®

  • Directly label primary antibodies with only 30s hands-on time, 2 hours total
  • Label primary antibodies, avoid laborious wash steps and cross-species reactivity
  • Site-specific, covalent labeling guarantees no interference with antibody performance
  • Compatible with all common buffers, even those containing Tris, azide & BSA
  • Highly homogeneous – no need to optimize conditions for each new antibody
  • Label from as little as 1 µg and work with antibody concentrations as low as 50 µg/mL
  • Store any unused reagents for later

oYo-Link® SureLight® Fluorescent Proteins

oYo-Link_PE_icon

oYo-Link® SureLight® PE

Exc/Emm: 565nm/574nm

Rapid, site-specific labeling of compatible antibodies with up to two PE labels. 30 seconds hands-on time, conjugates ready in just 2 hours.

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APC icon

oYo-Link® SureLight® APC

Exc/Emm: 653nm/660nm

Rapid, site-specific labeling of compatible antibodies with up to two APC labels. 30 seconds hands-on time, conjugates ready in just 2 hours.

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oYo-Link FR-APC

oYo-Link® SureLight® Far Red APC

Exc/Emm: 713nm/718nm

Rapid, site-specific labeling of compatible antibodies with up to two Far Red APC labels. 30 seconds hands-on time, conjugates ready in just 2 hours.

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oYo-Link PE-Cy7

oYo-Link® SureLight® PE-Cy7

Exc/Emm: 488 nm / 572 nm, 776 nm

Rapid, site-specific labeling of compatible antibodies with up to two PE-Cy7 labels. 30 seconds hands-on time, conjugates ready in just 2 hours.

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Two steps, 30 seconds hands-on time, 100% sample recovery

Antibody labeling requires just two steps:

  1. Mix oYo-Link with your antibody
  2. Illuminate with non-damaging 365 nm UV light for <2 hours

The entire procedure requires just two steps and less than 30 seconds of hands-on time. Any unused oYo-Link reagent can be saved for later use.

How oYo-Link® Works

oYo-Link reagents consist of low molecular weight (~8 kDa), high-affinity antibody-binding domains that possess a photo-crosslinker within their Fc-binding site. Upon illumination with non-damaging Black-light, oYo-Link forms a covalent bond with the antibody. This procedure is referred to as Light-Activated Site-Specific Conjugation (LASIC). Any label that is attached to oYo-Link will be covalently attached to the desired antibody.

 

All that is needed to label your antibody is the oYo-Link reagent and our LED photo-crosslinking device to perform the conjugation, or a compatible device.

Watch our webinar to find out more about oYo-Link.

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Looking to outsource your antibody conjugation?

AlphaThera also offers affordable, high quality antibody labeling performed by our expert team. Just $75 per conjugate plus the cost of reagents. Speak with our team about your project.

Talk to us about your project